John Tun Sein received his Diploma in Painting from the Sir J.J. School of Art, Mumbai, in 1985. Following his graduation, Sein received a Lalit Kala Akademi Research Grant Scholarship in 1988-89.
Sein's work has been featured in several solo exhibitions including those at Apparao Galleries, Chennai, in 2010; Gallery Art Motif, New Delhi, and Aicon Gallery, Palo Alto, in 2007; SWR, Heinrich/Stroebel-Haus, Baden-Baden, in 2005; Galerie Mueller and Plate, Munich, in 2000; and Pundole Art Gallery, Bombay, in 1999. He has also participated in several group exhibitions in India, Germany, Belgium, Poland, the UK and France.